ACHE and Hyperglycemia: Therefore, to determine whether AChE is elevated in diabetic zebrafish, the activity of AChE was estimated as detailed in materials and methods and the data in Figure 5B. Analysis of the data demonstrated that AChE activity was significantly increased in the hyperglycemia group (196.5 ± 5.98) when compared with the normal group (149.5 ± 4.61) (Figure 5B).