Downregulation of beclin1 and LC3 mRNA and protein expression is observed in response to the BV6 in combination with TRAIL and TNFα in both MCF7 and MDA-MB-231 breast cancer cell lines compared to the mRNA and protein expressions of biomarkers in the untreated group and individual treatments with TRAIL and TNFα. The gene discussed is MAP1LC3A; the disease is breast carcinoma.