Multifactorial Cox regression analysis indicated that gender, TNM stage, IGFBP1 and the IGFBP1hiWNT3Alo signature (p = 0.010; HR 0.520; 95% CI 0.380–0.803) was an independent predictor of prognosis in esophageal cancer (Figure 4B). The gene discussed is IGFBP1; the disease is esophageal cancer.