In particular, we reported that colon from BALB/c mice can efficiently be stimulated for 5 h with a range of concentrations of DNBS, ranging from 0.5 to 2.5 mg/mL, to induce the main hallmarks of IBD, such as decreased tissue permeability and altered morphology, induction of pro-inflammatory cytokines, and down-regulation of IL-10, in a dose-dependent manner. Here, IL10 is linked to inflammatory bowel disease.