We identified CpG sites associated with three imprinted genes, namely IGF-2, MEST, and KCNQ1, displaying aberrant methylation, but there were no alterations in the imprinted genes MEG3, KCNQ1OT1, and PEG3. Our results suggest that DNA methylation status may be associated with the poor semen parameters in infertile males. This evidence concerns the gene KCNQ1 and Infertility.