Quantifications of these stainings revealed significantly higher levels of phospho-tau (prefrontal cortex: 2957%; hippocampus: 2063%) and significantly reduced levels of NSun2 (prefrontal cortex: − 64.8%; hippocampus: − 9.1%) in neurons of the AD group (Fig. 3b, c, f, g) with an increased phospho-tau/NSun2 ratio at a cellular level (1806% in frontal cortex, 653% in hippocampus) (Fig. 3d, h). This evidence concerns the gene NSUN2 and Alzheimer disease.