More recently, CRISPR‒Cas9-based screenings in lung and colon cancer cell lines identified eukaryotic translation initiation factor 5B (eIF5B) and myeloid/lymphoid or mixed-lineage leukemia, translocated to 6 (MLLT6) as novel regulators of PD-L1 abundance, respectively7,8. Here, CD274 is linked to malignant colon neoplasm.