Simultaneous knockout of both TFEB and TFE3 (but not either gene alone) consistently decreased the proliferation of the TSC2 KO clones in in vitro confluence and viability assays (Fig. S4a, b), and also reduced in vivo growth of subcutaneous xenografts (Fig. 4b, c, Fig. S5), consistent with a role for MiT/TFE proteins as potential drivers of tumor progression in cells with constitutive mTORC1 activation. The gene discussed is TFE3; the disease is neoplasm.