We injected the mixture of AAV-mSncg-Cas9 + AAV-mouse HRH1-gRNAs or AAV-control gRNAs intravitreally into one of a mouse’s eyes five weeks before ONC (for traumatic ON injury model) or SO intracameral injection (SOHU glaucoma model) and used the contralateral eye as sham control (Fig. 7a). The gene discussed is HRH1; the disease is glaucoma.