Hippocampal sections were co-immunostained with EdU and antibody against DCX (doublecortin), a marker for newborn neurons derived from NSCs, as shown in Fig. S8A. Remarkably, TgAPPsweHSA mice at ages of 3-MO and 6-MO, but not 1-MO, displayed significant reductions in EdU+ and DCX+ cell densities at both dorsal and ventral DG (Fig. S8), demonstrating an early onset deficit in the NSC proliferation and thus DG neurogenesis in TgAPPsweHSA mice, exhibiting another similar deficit as AD animal models [40]. This evidence concerns the gene DCX and Alzheimer disease.