Although it was demonstrated that human glioma tumor (GBM) specimens are characterized by an upregulated cofilin pathway including elevated LIMK1/2 (relative to the normal brain) [224], and CuI-treated GBM cell lines led to multiple altered cellular outcomes (i.e., cytotoxicity, adhesion, migration, and invasion), no further mechanistic insights into cytoskeletal alterations induced by cucurbitacins were established, which would be a noteworthy future direction. This evidence concerns the gene CFL1 and glioblastoma.