Red fluorescence intensity decreased from 100 to 70% (± 11%) in PD-L1+ 786-O WT and PD-L1− 786-O KO cell lines over a 48 h period when NK-92 WT or PD1EcTM+ NK-92 cells were added to the tumor spheroids, with no significant differences between the three target cell lines. This evidence concerns the gene CD274 and neoplasm.