To find the ideal breast cancer cellular model to test the hypothesis that the inhibition of hypoxia-induced mitochondrial ROS production could result in the reversion of EMT, in addition to decreased HIF-1α stabilization, the three cell lines were characterized concerning their migratory capacity and ability to increase ROS and HIF-1α protein levels and to exhibit changes in EMT markers in response to hypoxia. This evidence concerns the gene HIF1A and breast carcinoma.