Finally, the effects of UQCRB downregulation on ROS production and HIF-1α and EMT protein levels were evaluated on MDA-MB468/pLVTHM, MDA-MB468/scrambledUQCRB and MDA-MB468/shUQCRB, obtained by infection of MDA-MB468 cells, as indicated in the Materials and Methods section, following 48 h incubation in the presence of 21% or 1% O2. This evidence concerns the gene HIF1A and infection.