In order to probe the immune microenvironment of the above two BCa clusters, we preliminarily analyzed the TCGA-BLCA cohort, and the results revealed that 62 ferroptosis regulators are positively correlated with PD-L1, 10 ferroptosis regulators are negatively correlated with PD-L1, and 26 ferroptosis regulators have no significant associations with PD-L1 (Figure 3A), this indicated that ferroptosis regulators may be tightly linked with the tumor immune microenvironment (TIME) of BCa. Here, CD274 is linked to bladder transitional cell carcinoma.