Currently, non-invasive tests such as long-non-coding RNA (Lnc RNA), neuron-specific enolase (NSE), circulating tumor DNA (ct DNA) and microRNAs have good diagnostic value for early diagnosis and cancer staging of lung cancer (11–14), however, there are few reports on the diagnosis of metastasis in non-small cell lung cancer. This evidence concerns the gene ENO2 and neoplasm.