In order to test the ability of human GNE cDNA to rescue the gne KO zebrafish from death, or ameliorate its symptoms, we generated a GNE Myopathy model by crossing the established transgenic line (described above, expressing the hGNE cDNA wild-type or M743T mutation under the control of zebrafish gne promoter) with the gne KO heterozygote fish to obtain an intermediate transgenic lineage. Here, GNE is linked to myopathy.