TP53 and metabolic dysfunction-associated steatohepatitis: In methionine-and choline-deficient diet-fed mice, serum IGF-1 levels decreased with the progression of simple steatosis to NASH, and the expression of p53 and its downstream target gene p21 in the liver also increased, which might be involved in initiating cell apoptosis and enhance clearance of damaged hepatocytes (Farrell et al., 2009).