Therefore, we suspected that CALN1 methylation was involved in the action of a DNase I hypersensitive site and, as a result, may affect the expression of CALN1. Regarding the relationship between bladder cancer and methylation, various analytical reports have centered on CpG sites [21, 22], and testing systems such as Bladder EpiCheck [23] have been established. Here, CALN1 is linked to urinary bladder cancer.