P1 had two separate oestrogen receptor (ER)-positive, human epidermal growth factor receptor 2 (HER2)-negative primary invasive breast cancers (PBCs) within a 5-cm bed of DCIS; we used tissue blocks from both PBCs (samples P1-ER1 and P1-ER2) and three regions from DCIS (samples P1-D1, P1-D2 and P1-D3). Here, ERBB2 is linked to ductal breast carcinoma in situ.