To demonstrate that BaSISS can chart these relationships across entire tissue sections, we examined three PBC samples with intermixed invasive and DCIS histology: P1-ER1, P1-ER2 and P2-TN1 (Fig. 3 and Extended Data Figs. 5 and 6a–c). Here, MIER1 is linked to ductal breast carcinoma in situ.