On the other hand, over-expression of either human TFPIβ (TFPI) or a mouse TFPIβ (mTFPI), but not TFPI2, elevated the sensitivity to TcdB4.2 in HeLa cells, and in a human bladder carcinoma cell line 5637 (a cell line that we found to be not sensitive to TcdB4.2) (Fig. 2g–i and Supplementary Fig. 6b). The gene discussed is TFPI; the disease is urinary bladder carcinoma.