Although PRDM1 single KO CAR T-cells demonstrated reduced Programmed Death-1 (PD-1) and TIM-3 expression in the peripheral blood, their inhibitory receptor expression was comparable to that of AAVS1 KO CAR T-cells in the tumor where CAR T-cells receive persistent antigen stimulation (Fig. 7D and E, fig. This evidence concerns the gene PPP1R12C and neoplasm.