In contrast, PRDM1/NR4A3 KO CAR T-cells demonstrated a significant reduction in the proportion of PD-1+TIM-3+ CD8 T-cells in both the tumor (P < 0.05) and peripheral blood as compared to AAVS1 KO CAR T-cells (P < 0.01, Fig. 7D and E). Here, NR4A3 is linked to neoplasm.