Both PRDM1 single KO and PRDM1/NR4A3 double KO CAR T-cells exhibited increased stem cell-like TIM-3−TCF1+ CD8 T-cells (45) in the tumor and peripheral blood, although only PRDM1/NR4A3 KO CAR T-cells showed a significant decrease in the frequencies of TIM-3+TCF1− exhausted T-cells in the tumor (P < 0.05, Fig. 7F and G, fig. The gene discussed is HAVCR2; the disease is neoplasm.