PRDM1 and neoplasm: Whereas AAVS1, PRDM1, and NR4A3 single KO CAR T-cells controlled tumor growth in about 50% of mice in the high-burden PC3-PSMA cell xenograft model, PRDM1/NR4A3 double KO CAR T-cells successfully suppressed tumor growth in all treated mice, in association with overall prolongation of survival (Fig. 7A and B).