B-type natriuretic peptide (BNP) and N-terminal pro-BNP (NT-pro-BNP), are important biomarkers for heart failure screening, diagnosis and differential diagnosis, assessment of disease severity and prognosis in the clinical practice, but their levels and efficacy are affected by age, the renal function, cardiovascular and non-cardiovascular diseases. The gene discussed is NPPB; the disease is cardiovascular disorder.