Interestingly, we also observed that co-stimulatory and co-inhibitory checkpoints were almost absent in CD8 + T cells except for the cycling cells (Fig. 4G), indicating that CD39i treatment did not completely reverse the tumor immunosuppressive microenvironment, that is, while CD39i enhances the cytotoxicity of cycling CD8 + cells, the anticancer function of these cells might also inhibited by other negative regulatory mechanisms, such as PD1/PD-L1 pathway. This evidence concerns the gene CD274 and neoplasm.