Our work provided a panoramic view of CD39i-mediated changes in the tumor microenvironment at the single-cell level, that is, CD39i noteworthily inhibited the tumor growth and improved the survival rate of mice by increasing the intratumor NK cells, cDC1 subpopulations, and CD8 + T cells, but decreasing Tregs. Here, MPPE1 is linked to neoplasm.