To determine if Myo1b could affect tumor growth and angiogenesis in vivo, particularly during tumorigenesis, HCT-116 cells stably transfected by NC or sh-Myo1b vector were injected orthotopically and subcutaneously in nude mice to establish the xenograft models and the transfection efficiency was confirmed by western blot (Fig. 6A). The gene discussed is MYO1B; the disease is neoplasm.