Through the integrative analysis of GEO data (GSE106582, GSE14333, GSE72970, GSE39084), we observed that Myo1b was obviously overexpressed in CRC tissues and its high expression was significantly related to poor prognosis of CRC patient, which was further validated in 8 matched human CRC and normal tissue specimens using western blot, RT-qPCR and IHC. The gene discussed is MYO1B; the disease is colorectal carcinoma.