To further examine whether wnt16 is a gene of major effect underlying pleiotropy at the CPED1-WNT16 locus, we generated germline mutants for cped1, as CPED1 is actively being investigated as a causal gene underlying effects at the CPED1-WNT16 locus on osteoporosis-related traits [49]. This evidence concerns the gene WNT16 and osteoporosis.