Moreover, the results of western blot displayed that a higher concentration of YQJPJD formula could remarkably reduce the protein expression levels of p-PI3K and p-Akt in HepG2 and Hep3B cells, indicating that YQJPJD formula may influence the proliferation, apoptosis, migration, and invasion of HCC cells by inhibiting the activation of PI3K/Akt pathway. This evidence concerns the gene PIK3CA and hepatocellular carcinoma.