Although there is an mRNA signal for the NEFL transcript for RWPE-1 cells which is a normal epithelial prostate cancer cell line (Figure 1A), the protein is translated and expressed at extremely low levels (Figure 1C), and with >5 fold upregulation in PC3-ML2 versus RWPE1-1.NEFL is not expressed in the other cell lines which include the androgen dependent LNCAP and its derivative C4-2 cell lines. The gene discussed is NEFL; the disease is prostate carcinoma.