PAH ECFCs from the two-hit ECFC PAH model (combining PAH BMPR2 mutations and hypoxia) showed 2360 DEG, while HPASMCs showed 689 DEG (logFC > 0.25, P < 0.05) (Fig. 4f, g and Supplementary Data 1). The gene discussed is BMPR2; the disease is pulmonary arterial hypertension.