Transcriptional profiling of HPAECs from the two-hit model identified multiple genes linked with known PAH pathways, including TGF-β signalling, NOS pathway, proliferation, angiogenesis, Notch signalling, EndoMT, ion channels, fibrosis, and inflammation (Fig. 3a and Supplementary Fig. 10a). The gene discussed is TGFB1; the disease is pulmonary arterial hypertension.