SRSF5 was downregulated in macrophages and Treg cells from pN+ HNSCC in relation to pN0 samples, and this sense of alteration is in accordance with the results obtained in proteomics (Fig. 5b) (adjusted p ≤ 0.05; two-sided Wilcoxon test and Benjamini-Hochberg correction). The gene discussed is SRSF5; the disease is head and neck squamous cell carcinoma.