We then knocked down Snail in SIRT2-overexpressing cells (Fig. 8G) and showed that Snail knockdown abrogated the promoting effect of SIRT2 on the migration and invasion of osteosarcoma cells (Fig. 8H, I), suggesting that SIRT2 promotes the invasion and metastasis of osteosarcoma cells via Snail. This evidence concerns the gene SIRT2 and osteosarcoma.