To clarify the regulatory relationship between SIRT2 and Snail, we detected the expression of Snail in osteosarcoma cells, and Snail expression was upregulated in MG63 and Saos-2 cells (with high SIRT2 expression) compared with hFOB1.19 control cells (with low SIRT2 expression) (Fig. 7C). This evidence concerns the gene SNAI1 and osteosarcoma.