De novo DNA methylation is acquired by PD1+CD8+ tumor-infiltrating T cells (TILs), which results in graded downregulation of cytokines, such as interferon-γ (IFN-γ), while 5-Aza-2’-deoxycytidine (DAC) de novo blocks DNA methylation in activated PD1+CD8+ TILs (147). The gene discussed is CD8A; the disease is neoplasm.