Results showed that the markers for activation T cells including interleukin-2 receptor alpha chain (IL2RA, or CD25), CD69, CD8a, CD28, check point inhibitors PD-L1 and CTLA4, and T cell exhaustion marker Tim-3 were all significantly enhanced in the tumor from the apoE-/- mice in which apoE-/- cells were inoculated (apoE-/-/apoE-/-) compared with control group (wt/wt), PD1 and Lag 3 were slightly increased, but they were not statistically significant (data not shown). The gene discussed is CD28; the disease is neoplasm.