In a mouse CCl4 liver fibrosis model, EVs from RLN-preconditioned mouse macrophage cell lines (Raw264.7) showed higher potency to lower serum ALT/aspartate aminotransferase (AST) and reduced liver fibrosis (hydroxyproline and α-SMA levels) than native EVs (153). The gene discussed is ACTA1; the disease is Hepatic fibrosis.