After excluding unrelated pathways, such as “human cytomegalovirus infection,” “tuberculosis” and “hepatitis B,” the top 10 signaling pathways with the lowest p value were selected (Figure 6A), among which, the PI3K-Akt, TNF, IL-17 and HIF-1 signaling pathways were correlated with inflammation and immunity, while apoptosis, Alzheimer’s disease, pathways of neurodegeneration-multiple diseases, and dopaminergic synapse were closely related to the pathogenesis of AD. Here, AKT1 is linked to early-onset autosomal dominant Alzheimer disease.