Neuropeptides participate in trigeminal activation, which is believed to be a crucial stage in the processing of migraine pain, including calcitonin gene-related peptide (CGRP), substance P (SP), and pituitary adenylate cyclase-activating polypeptide (PACAP) (Pietrobon and Moskowitz, 2013; Zagami et al., 2014; Iyengar et al., 2019). Here, ADCYAP1 is linked to migraine disorder.