In critically ill patients there was a seven fold increase in risk of AKI in patients with a [TIMP-2]× [IGFBP7] value of >0.3 ng/ml when compared to those with a score ≤0.3 ng/ml (99) making this a possible acceptable cut off for assessing for increased AKI risk in critically ill patients. The gene discussed is TIMP2; the disease is acute kidney injury.