Statistical results showed that GFAP and FABP-2 at birth significantly associated with increased risk of NEC with OR = 15.629 (95% CI = 1.697–143.906), P = 0.015 and OR = 1.008 (95%CI = 1.001–1.015), P = 0.033, respectively. This evidence concerns the gene GFAP and necrotizing enterocolitis.