We first verified the transfection efficiency of colon cancer cells by qRT-PCR (Figure 4(a)) and found that FXR overexpression partially offsets the increase of cell proliferative ability and colony forming ability induced by DHRS9 silencing in colon cancer cells (Figure 4(b) and 4(c)). This evidence concerns the gene NR1H4 and malignant colon neoplasm.