SOD1 and amyotrophic lateral sclerosis: Specifically, Li et al. used H2O2 to treat G93A-SOD1 transfected NSC34 cells as a cellular model of ALS and found that celastrol reduced cell death, involving activation of mitogen-activated protein kinase (MEK)/extracellular regulated protein kinases (ERK) and phosphoinositide 3-kinase (PI3K)/serine-threonine kinase (AKT) signaling pathways (Li et al., 2017).