What’s more, correlation analysis between the m7Gscore and immune checkpoint molecules in each cancer was also conducted and we found most immune checkpoint molecules were negatively correlated with the m7gscore across cancers, except for CD274 in KIRP, KIRC and KICH, CD276 in PRAD and ESCA, ICOSLG in KIRP and KIRC, IL-1A in HNSC, SIGLEC15 in KICH and TNFSF4 in PRAD (Supplementary Figure S3C). The gene discussed is ICOSLG; the disease is prostate adenocarcinoma.