In HCT116 cells, SW480 cells, and DSS-induced CRC mice, ursodeoxycholic acid (UDCA), as one of the main active components of bile, inhibits the malignant progression of colorectal cancer through TGR5-mediated cAMP-PKA-RhoA signaling pathway antagonizing YES-associated protein9. Here, GPBAR1 is linked to colorectal cancer.