Bioinformatics analysis suggested that the downstream target genes of LncRNA HOTAIR were miR-519b-3p and Prrg4. Further studies indicated that LncRNA HOTAIR regulates cell invasion and migration in endometriosis by regulating the miR-519b-3p/PRRG4 pathway, which may prove to be potential markers and new targets for early diagnosis and treatment of endometriosis. This evidence concerns the gene PRRG4 and endometriosis.