FLI1 and Ewing sarcoma: Although FISH, IHC and/or RT-PCR are still the most frequently used diagnostic adjunct (i.e. EWS RNA binding protein 1 (EWSR1)-friend leukemia virus integration 1 (FLI1) fusion in Ewing sarcoma), the introduction of next-generation sequencing (NGS) technologies has dramatically improved the scenario, particularly in cases requiring multiple testing, where NGS is cost-effective in identifying known and novel fusions genes [6, 7].