After isolation of EVs from human plasma in patients with CAD spun at 20,000 g, PAI-1+ PEVs (defined as Annexin V+/CD41+/PAI-1+ large EVs) were measured by using flow cytometry after generating a size gate using standardized Apogee beads (Apogee Flow Systems). Here, ANXA5 is linked to coronary artery disorder.