Similar to the in vivo experiments, IRF7 expression levels in colonic ECs were decreased by the infection of adenovirus-packaged sh-IRF7 (p < 0.01, Figure 4A and 4B), after which the cell viability was increased (p < 0.01, Figure 4C), ROS levels diminished, GSH levels increased (p < 0.01, Figure 4D and 4E), GPX4 expression levels elevated, SLC11A2 protein expressions decreased (p < 0.01, Figure 4B), iron ion levels decreased (p < 0.01, Figure 4F), and the cell lipid peroxidation was reduced (Figure 4G). Here, IRF7 is linked to infection.