KLF13 and gastric cancer: Therefore, to further confirm the effect of KLF13 on gastric cancer, plasmid expressing KLF13 gene was transfected into two gastric cancer cell lines, BGC-823 and SGC-7901, and transfection efficiency was detected by Western blot in Fig. 2C. Then, colony formation assay, CCK-8 assay, and cell cycle analysis using flow cytometry were performed respectively to test capability of cell proliferation of the indicated gastric cancer cell lines which were transfected with KLF13 plasmid.