Considering the high expression of CD123 on AML blasts, we generated a retroviral vector encoding a second-generation CAR.CD123.41bb.CD3z (Additional file 1: Fig. S3A) to genetically modify primary NK cells purified from either the BC or leukapheresis of HDs (PB-NK). The gene discussed is IL3RA; the disease is acute myeloid leukemia.