In this study, we used TCGA and GEO databases to conduct pan-cancer analysis of HK2, including the expression of the HK2 gene in different tumors, the effect of HK2 on tumor survival, immune-infiltrating cells and related cellular pathway changes, etc., to explore the potential molecular mechanism of HK2 in the pathogenesis or clinical prognosis of different tumors. Here, HK2 is linked to neoplasm.