The present data showed that there are some differences in protein conformational changes, stability, glycolysis, mitochondrial oxidative phosphorylation, and PI3K/AKT signaling among these IDH2 point mutations, specifically for the most common IDH2 point mutations R140Q and R172K in cancer cells, indicating that each IDH2 point mutation possibly plays a different biological role in tumorigenesis. The gene discussed is IDH2; the disease is cancer.