To test whether PITX1 destabilizes cancer cell adhesion to the ECM, we performed cell adhesion assays by seeding control or PITX1-overexpressing OS cells in plates coated with defined components of the ECM, i.e., fibronectin, laminin, and collagen I. The results showed that PITX1 inhibited cell adhesion of OS cells (Fig. 2C). This evidence concerns the gene FN1 and cancer.